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The 2nd response cycle in NO synthesis includes O2 binding to ferrous NOS to form the Fe, IIO2 intermediate, followed by oxidation of NOHA to produce ferric enzyme, NO, citrulline, and water as products (Fig. 9) - UV/Vis. As attracted Fig - https://www.livebinders.com/b/3570027?tabid=514355ed-03f4-acee-f8e7-d79f6b7bffab. 9, the response may also include electron transfer from H4B to the Fe, IIO2 intermediate

l9 As displayed in Fig. 9, this could be since the H4B radical acts as an electron sink in a later point in the response, so regarding allow NOS to, Fig. 9. A design for NORA oxidation by NOSoxy in the single turnover reaction. Oxygen binds to the ferrous protein to form the Fe, IIO2 species, which upon getting an electron from H4B forms a putative hemeperoxo intermediate that responds with NOHA.